ENUCLEATED NEUROBLASTOMA CELLS FORM NEURITES WHEN TREATED WITH DIBUTYRYL CYCLIC AMP
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منابع مشابه
Enucleated Neuroblastoma Cells Form Neurites When Treated with Dibutyryl Cyclic Amp
Cultured cells cloned from the C1300 neuroblastoma have been shown by several laboratories to extend neurites (1-6) and to accumulate nervespecific enzymatic activities (7) when grown under conditions in which cell division is inhibited. Study of this system has been stimulated by the hope that it might serve as an in vitro model of nerve cell differentiation. Many of the neurite-inducing agent...
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Blot hybridization analysis that used labeled tubulin cDNA probes revealed that N6,O2'-dibutyryladenosine 3',5'-cyclic monophosphate [dibutyryl cyclic AMP (Bt2cAMP)] initially increases and later decreases the level of tubulin mRNA in a neuroblastoma-glioma hybrid cell line as well as in the parent cells. A significant increase in tubulin mRNA sequences is already evident 1 hr after the additio...
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Sodium and calcium inward currents (INa and ICa) were measured in neuroblastoma X glioma hybrid cells of clones 108CC5 and 108CC15 by a single suction pipette method for internal perfusion and voltage clamp. Morphologically undifferentiated, exponentially growing cells were compared with cells differentiated by cultivation with 1 mmol/l dibutyryl cyclic AMP. Outward currents were eliminated by ...
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The intermixing of chick embryonic heart ventricle cells was studied in cellular aggregates as a function of embryonic age of the cells. Cell mobility, as measured by intermixing of radiolabelled and unlabelled cells, was high in cells derived from young embryos: 5.36 +/- 1.05 cell diameters per 2.5 days for 6-day-old heart ventricle cells. During development, mobility steadily declined to 1.56...
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Dibutyryl cyclic AMP was administered to 2 patients with severe congestive heart failure. After an intravenous drip infusion of dibutyryl cyclic AMP (300 mg/40 min), marked diuresis and mild improvement of the patients' physical activity occurred. The cardiac index increased and both systemic vascular resistance and pulmonary capillary wedge pressure decreased. Blood glucose and plasma insulin ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1974
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.63.1.295